Equipements et instruments de laboratoire, AMA E60PV-19EQUI

Procédure d’appel d’offre : Invitation à soumissionner suivant les instructions de la AMA
Stratégie d’approvisionnement concurrentiel : Contrat subséquent à la AMA sera offert au fournisseur répondant aux critères techniques obligatoires et aillant le prix le bas.
Entente sur les revendications territoriale globale : Non
Nature du besoin :
Équipement et Instrument de Laboratoire, AMA E60PV-19EQU
Pour :
L’achat d`une (1)Plateforme de pipetage robotique et un (1) système de manipulation de liquides suivant les spécifications indiquées sur l’appel d’offres
Agriculture et Agroalimentaire Canada, Centre de services de l’Est
CETTE DEMANDE EST RÉSERVÉ POUR LES TITULAIRES DE L’ARRANGEMENT EN MATIÈRE D’APPROVISIONNEMENT # E60PV-19EQUI SEULEMENT, POUR L’ACHAT D’ÉQUIPEMENT, DE PIÈCES ET ACCESSOIRES, DE FOURNITURES ET DE SERVICES DE LABORATOIRE ET SCIENTIFIQUE.
Seulement les fournisseurs présentement pré-qualifiés sur l’arrangement en matière d’approvisionnement E60PV-19EQUI ont été invité à soumettre une soumission.
Conformément à l’arrangement en matière d’approvisionnement, cet avis est publié sur Achat et Vente Canada pour une période de 40 jours civils. La date de clôture publiée sur cet avis indique sa durée de publication. Pour connaître la date de clôture de toute demande de soumissions dans le cadre de l’arrangement en matière d’approvisionnement, les fournisseurs invités doivent se reporter aux documents de sollicitation.
Les fournisseurs qui n’ont pas d’arrangement en matière d’approvisionnement avec Travaux publics et Services gouvernementaux Canada pour l’équipement, pièces et accessoires, fournitures et services de laboratoire et scientifique ne peuvent soumissionner. Toutes offres reçues de fournisseurs non pré-qualifiés dans l’arrangement en matière d’approvisionnement ne sera pas évaluée.
Les fournisseurs peuvent, à tout moment, se qualifier sous l’arrangement en matière d’approvisionnement E60PV-19EQUI pour l’équipement, pièces et accessoires, fournitures et services de laboratoire et scientifique. Les fournisseurs intéressés doivent télécharger le document de sollicitation E60PV-19EQUI/C sur achatsetventes.gc.ca et soumettre une réponse selon les exigences de la demande d’arrangement en matière d’approvisionnement.
Besoin :
Le Centre de recherche de Harrow, Agriculture et Agroalimentaire Canada, a un besoin pour faire l’acquisition d`un (1) Plateforme de pipetage robotique et un (1) système de manipulation de liquides
Les critères techniques obligatoires sont :ATTENTION ANGLAIS
Table 1. Micro-volume liquid handling robot for soil DNA extraction lab
Mandatory Technical Criteria
a.
The system must be fully enclosed on all sides and measure a maximum of 65cm (26) L x 61cm (24) W x 67cm (27) H when closed.
b.
The system must have a safety door with pausing of operation if the door is opened
c.
The system must include a worktable with a minimum of 6 positions.
d.
The system must have a UV/HEPA cap, providing the option of UV light (254 nm) for decontamination and HEPA filtration (70 – 80 m³/h) to provide clean air inside of the system
e.
The system must provide contact free detection of liquid levels, of dispensing tools and of system accessories, labwares, as well as tip size and quantity. This contact free detection must be done by measuring the light reflection of surfaces. The system must not require electrically conductive tips.
f.
The system must operate with liquid handling tools (pipettes) that offer a pipetting range of 0.2µl to 1000µl with the potential to be autoclaved. The system must have options to pipet this range with both single-channel tool and 8-channel tools. At any given time, up to 2 dispensing tools must be available for use during a program (without user intervention), or up to 8 (with user intervention).
g.
Dispensing tools must be autoclavable, have automatic calibration warnings, and be calibrated based on ISO 8655-6.
h.
The system MUST be able to provide contact free dispensing of samples from the pipette tip into labware, AS WELL AS being able to allow exact positioning of the pipet tip relative to liquid meniscus or labware bottom during aspiration and dispensing.
i.
The system must be free of a backfilled system liquid, and utilize air-displacement pipetting tools.
j.
The system must be an open platform system and be compatible with any commercially available labware (plates, tubes, etc). To this end, the software must possess a database of predefined labware and consumables for all components used on the system, including for Eppendorf twintec plates used in lab downstream applications. Predefined labware includes: Tubes, equipment racks, thermoblocks, thermoadapters, thermoracks, 96 and 384 well microplates and Deep Well plates, reservoirs, height adapters and tubs among other consumables and labware. This database allows the user to avoid adjusting offsets and measuring positioning on the deck, and thus streamline application programming.
k.
The control and operating software must include the following functions: touch panel with a minimum of 2 front USB ports; a set of pre-installed PCR wizards/assistants that will guide the user through the programming of the automation process by answering simple questions, including transferring and mixing late stage DNA extraction reagents and normalization of samples; allow CSV or XLM file exchange via USB key or LAN; must give users the ability to rinse tips before ejecting the tips into the waste; must be able to re-load/re-use tips during a program; must allow dipping of the tip into the target vessel in order to remove any hanging drops after dispensing; must allow tip piercing of adhesive foil on 96 and 384 well plates; must have integrated plastic-ware selection tools to reduce time associated with programming parameters; must have copy/ paste commands to copy commands and labware from one application to the other.
l.
The system must include a single-channel dispensing tool with volume capacity of 0.2-10 µL and an 8-channel dispensing tool with volume capacity of 1-50 µL
m.
The system must include the following accessories: a thermorack for 384-well PCR plates, reagent and reservoir racks
n.
The system must include start-up consumables, including PCR-clean filter tips, reservoirs, and waste bags.
o.
Accessories including all tools and consumables must be cross-compatible with the next generation sequencing system outlined in Table 2, in order to maximize operability and reduce overall costs.
p.
Delivery must be complete before March 31, 2021. The supplier must commit to installation within the time-frame outlined below.
q.
Onsite and/or remote training must be included within the time-frame outlined below.
Table 2. Robotics for NGS and related RNA-protocol applications
Mandatory Technical Criteria
a.
The system must be fully enclosed on all sides, measure a maximum of 107cm (43) L x 61cm (24) W x 67cm (27) H when closed
b.
The system must have a safety door with pausing of operation if the door is opened
c.
The system must include a worktable with a minimum of 15 positions.
d.
At least one of the 15 deck positions must include a thermo-mixing module that is fully integrated in the worktable and controlled via the system’s software. The module must combine 2D orbital shaking with heating and cooling technology and must be able to operate while simultaneously pipetting onto other worktable positions. Minimum specifications must be as follows: Mixing range 300 to 2,000 rpm, Temperature range: 4°C from room temperature to 95°C, Heating rate: 5°C/min, Cooling rate: 3°C/min.
e.
The system must contain at least one (1)
magnetic bead purification plate position, each containing strong magnets
f.
The system must contain a microplate gripper,to assist with automated consumable management
g.
The system must have the option of including a UV/HEPA cap, providing the option of UV light (254 nm) for decontamination and HEPA filtration (70 – 80 m³/h).
h.
The system must provide contact free detection by measuring the light reflection of surfaces of liquid levels, of dispensing tools and of system accessories, labwares, as well as tip size and quantity
i.
The system must operate with pipettes that offer a pipetting range of 0.2µl to 1000µl. The system must have options to pipet this range with either a single-channel tool or an 8-channel tool. At any given time, up to 4 dispensing tools must be available for use during a program (without user intervention).
j.
Dispensing tools must be autoclavable, have automatic calibration warnings, and be calibrated based on ISO 8655-6.
k.
The system must provide the following pipetting precision: Random measurement error (%) for small volumes (1µl) < 3% and larger volumes (1000µl) < 0.15% relative to pipette size.
l.
The system must be free of a backfilled system liquid, and utilize air-displacement pipetting tools.
m.
The system must be able to provide contact free dispensing of samples from the pipette tip into labware, as well as be able to allow exact positioning of the pipet tip relative to liquid meniscus or labware bottom during aspiration and dispensing.
n.
The system must provide a gripper arm to move labware to and from any position on the worktable. This would be used to move labware from Thermo-mixing element to a plate magnet during NGS library preparations methods.
o.
The system must be an open platform system and be compatible with commercially available labware (plates, tubes, etc). To this end, the software must possess a database of predefined labware and consumables for all components used on the system, including for Eppendorf twintec plates used in lab downstream applications. Predefined labware includes: Tubes, equipment racks, thermoblocks, thermoadapters, thermoracks, 96 and 384 well microplates and Deep Well plates, reservoirs, height adapters and tubs among other consumables and labware.
p.
The system must be compatible with any commercially available NGS library preparation kit. Furthermore, any existing or future optimized protocol must be available free of charge and must be readily available to download without restriction for as long as the instrument will be in use
q.
The control and operating software must include the following functions: touch panel with a minimum of 2 front USB ports; a set of pre-installed PCR wizards/assistants that will guide the user through the programming of the automation process by answering simple questions; wizards/assistants must include standard Illumina library preparation protocols (i.e. TruSeq DNA PCR-Free); allow CSV or XLM file exchange via USB key or LAN; must allow dipping of the tip into the target vessel in order to remove any hanging drops after dispensing; must have integrated plastic-ware selection tools to reduce time associated with programming parameters; must have copy/ paste commands to copy commands and labware from one application to the other.
r.
The system must include single and 8-channel 50 ul and 300 ul dispensing pipettes
s.
The system must include the following NGS specific accessories: 96 well thermoblock, 2 x 96 well thermoadapter, tube rack, reservoir racks, magnetic adaptors, and 400 ml waste bag holders.
t.
The system must include start-up consumables, including PCR-clean 50 and 300 ul filter tips, reservoirs, and waste bags.
u.
Accessories including all tools and consumables must be cross-compatible with the DNA liquid handling outlined in Table 1, in order to maximize operability and reduce overall costs.
v.
Delivery must be complete before March 31, 2021. The supplier must commit to installation within the time-frame outlined below.
w.
Onsite and/or remote training must be included within the time-frame outlined below.
Le soumissionnaire répondant déjà titulaire d’un arrangement en matière d’approvisionnement pour l’équipement, pièces et accessoires, fournitures et services de laboratoire et scientifique de Travaux publics et Services gouvernementaux Canada, qui répond au critères techniques obligatoires avec le prix global le plus bas sera recommandé pour l’attribution du contrat.